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human shrna plasmid kits for ron  (OriGene)


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    OriGene human shrna plasmid kits for ron
    Figure 4: <t>RON</t> kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R <t>shRNA</t> constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
    Human Shrna Plasmid Kits For Ron, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ron+sirna/RON+(MST1R)+Human+shRNA+Plasmid+Kit/pm36537918-135-2-18
    Average 91 stars, based on 1 article reviews
    human shrna plasmid kits for ron - by Bioz Stars, 2026-08
    91/100 stars

    Images

    1) Product Images from "Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms."

    Article Title: Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    doi: 10.1158/1078-0432.CCR-22-1763

    Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or
    Figure Legend Snippet: Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or

    Techniques Used: Western Blot, Expressing, Transduction, shRNA, Construct, Colony Assay, Comparison, Phospho-proteomics, Flow Cytometry, Control, Ex Vivo, Retroviral, Transplantation Assay



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    Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms.

    doi: 10.1158/1078-0432.CCR-22-1763

    Figure Lengend Snippet: Figure 4: RON kinase is an important target of crizotinib in MPN (A) Western analysis of RON in SET2 cells treated with crizotinib and stimulated with erythropoietin (EPO), stem cell factor (SCF), and hepatocyte growth factor (HGF). Representative data from one of at least three independent experiments is shown (B) qPCR of MST1R (RON) expression in HEL cells transduced with 4 different MST1R shRNA constructs (C) Colony formation assay of transduced HEL cells in methylcellulose with myeloid growth factors. Experiments were performed at least three times, with similar results. ANOVA with Tukey’s multiple-comparison test was used. (D) Western analysis of RON, STAT3 and STAT5 phosphorylation in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF. Representative data from one of three independent experiments are shown. (E) Western analysis of JAK2 in shRNA-transduced HEL cells stimulated with EPO, SCF, and HGF (F) Flow cytometry analysis of RON phosphorylation in MPN patient or control donor peripheral blood CD45lo population with ex vivo EPO and SCF stimulation (G) Schematic for JAK2-V617F-GFP retroviral bone marrow transplantation using bone marrow from Stk−/− (RON−/−) mice (H) Peripheral blood analysis of WT (N=8) or Stk−/− (N=9) JAK2-V617F mice over time. WBC= white blood cells; Hb=hemoglobin; PLT=platelets (I) Percentage of GFP in the peripheral blood over time in WT (N=8) or

    Article Snippet: Lentiviral Production: Human shRNA plasmid kits for RON (Cat # TL320425) and MET (Cat #TL320418) were purchased from Origene.

    Techniques: Western Blot, Expressing, Transduction, shRNA, Construct, Colony Assay, Comparison, Phospho-proteomics, Flow Cytometry, Control, Ex Vivo, Retroviral, Transplantation Assay